Expression

Accelerate Your Antibody Discovery Program

Antibody Expression

To meet the rigorous timelines of modern biologics development, Invenra’s B-Body Express™ service provides a rapid turnaround from DNA to purified antibodies, delivering high-quality results in as little as 4 to 8 weeks. This service is designed to de-risk therapeutic pipelines by offering flexible production scales of 50mL, 200mL, or 500mL, allowing for a seamless transition from small-scale high-throughput screening to larger validation studies. By delivering high-purity monoclonal and B-Body® bispecific antibodies with no material caps and flexible licensing options, Invenra provides the high-quality material and comprehensive QC data necessary to confirm candidate viability and accelerate the entire antibody discovery and development process.

Fast-track your therapeutic antibody development today—contact us to learn more!

Expressed Antibody Results High-yield, high-purity B-Body Bispecific Antibody purified by one-step Protein A purification following transient expression CHO cells, demonstrating stability and purity in reducing and nonreducing PAGE.

Features:

• Inputs: Your antibody sequences
• Deliverables:
  • Two-step purified mAb or B-Body Bispecific Abs
  • QC analysis of expressed antibodies
• Timeline – 4 weeks from DNA construction to antibody shipment
• Flexible Scale – Choose 50mL, 200mL or 500mL expression

Process:

1. Design & Quote – Collaborate with our team to select from our validated mAb or B-Body formats, desired sample quantiy and expression scale
2. Express & Purify – We prepare DNA constructs, express, and 2-step purify your antibodies for high purity
3. Analyze & Deliver – Receive QC analyzed Abs — with no retention limits (you receive the entire yield of Ab)

Advantages:

• Seamless B-Body Integration– Test bispecifics alongside traditional mAbs
• Flexible Formats – Choose from mAb or 1×1 formats
• Three Production Scale Options – Select between 50mL, 200mL or 500mL expression scales and all produced antibody is yours
• Full Service Production – Invenra manages all aspects of antibody production from DNA ordering, expression, and two-step purification and characterization
• Rapid Antibody Availability – Fully purified material is delivered in 4 weeks from DNA construction
• Flexible Commercial Licensing Options – Please contact us to discuss pricing and terms

Example Expression Performance

93% Purity After One Step Protein A Purification

HPLC analysis illustrates the high yield and-purity possible with B-Body® Bispecific antibody expression and one-step purification. Bispecific Ab was transiently expressed in CHO cells and purified by Protein A purification. HPLC analysis provides readouts of yield and purity as well as valuable, predictive insights for analytical development, downstream processing, and formulation optimization.

High Performance Across a Range of Formats and Samples

This dataset comprises 549 protein samples (~493 unique sequences) expressed in CHO at 50–200 mL scale, spanning IgG1, One Arm, 1×1, 2×1, 2×2, and trispecific formats.

Panel A shows crude yield after capture purification (x-axis) versus crude purity (y-axis), illustrating that the service can successfully express antibodies across a broad range of molecular formats and a large range of antibody specificities.

Panel B summarizes purity distributions for crude and polished samples. In each box plot, the central line represents the median, the box edges mark the 25th and 75th percentiles, and whiskers indicate the statistical minimum and maximum values (outliers excluded). In all cases, polishing improved purity, with the lowest observed values exceeding 85%, demonstrating that the service reliably delivers high-quality material across diverse antibody architectures.

Get Started with Invenra Antibody Discovery & Expression

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